Composite

Part:BBa_K3385059:Design

Designed by: Daniel Bavnh�j   Group: iGEM20_DTU-Denmark   (2020-10-16)


T1G3R_SpmeA_glaA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5750
    Illegal EcoRI site found at 7178
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5781
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4012
    Illegal PstI site found at 6588
    Illegal PstI site found at 8322
    Illegal PstI site found at 8923
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5750
    Illegal EcoRI site found at 7178
    Illegal NheI site found at 4889
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4012
    Illegal PstI site found at 6588
    Illegal PstI site found at 8322
    Illegal PstI site found at 8923
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5750
    Illegal EcoRI site found at 7178
    Illegal BglII site found at 3867
    Illegal BglII site found at 5389
    Illegal BglII site found at 8420
    Illegal BamHI site found at 1729
    Illegal BamHI site found at 2157
    Illegal BamHI site found at 4847
    Illegal BamHI site found at 5649
    Illegal BamHI site found at 5775
    Illegal BamHI site found at 6326
    Illegal XhoI site found at 548
    Illegal XhoI site found at 735
    Illegal XhoI site found at 963
    Illegal XhoI site found at 6186
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5750
    Illegal EcoRI site found at 7178
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5781
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4012
    Illegal PstI site found at 6588
    Illegal PstI site found at 8322
    Illegal PstI site found at 8923
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1858
    Illegal EcoRI site found at 5750
    Illegal EcoRI site found at 7178
    Illegal XbaI site found at 1623
    Illegal XbaI site found at 5781
    Illegal SpeI site found at 1097
    Illegal PstI site found at 893
    Illegal PstI site found at 4012
    Illegal PstI site found at 6588
    Illegal PstI site found at 8322
    Illegal PstI site found at 8923
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 5107
    Illegal NgoMIV site found at 8310
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3395
    Illegal BsaI site found at 3533
    Illegal BsaI site found at 4222
    Illegal BsaI.rc site found at 4715
    Illegal SapI site found at 8811
    Illegal SapI site found at 9120


Design Notes

Full construct made using non-overlapping primers, which can pose difficulties on PCR amplification and subsequently USER assembly.

Source

Sequences were obtained using aspgd.org[1] and SingalP-5.0[2].

References

[1] Cerqueira GC, Arnaud MB, Inglis DO, Skrzypek MS, Binkley G, Simison M, Miyasato SR, Binkley J, Orvis J, Shah P, Wymore F, Sherlock G, Wortman JR (2014). The Aspergillus Genome Database: multispecies curation and incorporation of RNA-Seq data to improve structural gene annotations. Nucleic Acids Res 42 (1); D705-10.

[2] SignalP 5.0 improves signal peptide predictions using deep neural networks. José Juan Almagro Armenteros, Konstantinos D. Tsirigos, Casper Kaae Sønderby, Thomas Nordahl Petersen, Ole Winther, Søren Brunak, Gunnar von Heijne and Henrik Nielsen. Nature Biotechnology, 37, 420-423, doi:10.1038/s41587-019-0036-z (2019)